Biochemistry lab report Example | Topics and Well Written Essays - 2500 words - 1

Biochemistry - Lab Report Example Paper chromatography is actually liquid-liquid chromatography, the paper should not be considered as solid phase, but the water molecules trapped in the cellulose of the paper form the ‘stationary’ phase. To saturate the cellulose, most paper chromatographic solvents have some amount of water in it. So the components with very high water solubility will move slower ( Parà © and Bà ©langer, 1997). Method: A 60:40 v/v acetonitrile: ammonium ethonate mobile phase is made, pH 7.2, and placed in a covered tall jar. Aqueous solutions of amino acids are spotted on the specified location (origin) on the stationary phase using a capillary and allowed to dry. The stationary phase is then put into the jar with mobile phase and allowed to run for 40 minutes. Mark the solvent front. Make sure the solvent stays well below the top edge. The stationary phase is then dried and sprayed with ninhydrin solution in the fume hood, and heated to allow the color to develop. From the Rf values (Table 1) it seems neither molecular weight nor the polarity had any significant effect on the migration. Glycine being the smallest did not travel the farthest. Looking at both, Rf and the color developed the sample X is Lysine and Y is Proline. Finger prints are seen on both the lateral sides of the paper, probably at the places used to handle the paper. They appear due to reaction between ninhydrin and the terminal amines of the lysine de-bonded from the amino acid. Also the sweat-gland secretions in the ridges of the fingers has proteins too (Sens,Simmons and Spicer, 1985). Conclusion: Paper chromatography can be used to separate amino acid from a mixture of amino acids. The migration of amino acids on the solid phase is a complex interplay between the molecular eight, shape, structure and polarity of the amino acids and their affinity towards the solid and the mobile phase. Use of ninhydrin to identify amino

Business Plan (Restaurant) Essay Example | Topics and Well Written Essays - 2500 words

Business Plan (Restaurant) - Essay Example The main logistics issued discussed include outlining the processes for opening a restaurant in London, recruitment of workers, location of the restaurant and estimating competition from other restaurants in the area. Some possible future problems are highlighted and solutions needed to tackle them are presented. For example, the issue of credit or financial crunch is elaborately discussed and the managerial methods required to steer the restaurant into financial survival are clearly explained in the report. Setting up a restaurant in London City might be an interesting investment based on the teeming number of people that live in the city. London has been regarded as the most multicultural city in the entire European bloc: Most London residents come from a variety of backgrounds rich in local cultures and cuisines (Wilson, 2006). The importance of this status is that any restaurant opened in the city will definitely find buyers for its kinds of foods and drinks, be it African, Asian, European or Native American. As the cultural centre of the United Kingdom, London City also attracts a host of visitors from all over the world. These are potential customers who are willing and ready to order for any dishes that serve their best interest. This, in essence, is the most interesting part of the Cultural Revolution through diversity that London has been promoting for ages (Parijs, 2004). Starting a restaurant in London may not be an easy task: but, it could bring a satisfactory result if it is properly undertaken. There are some factors to consider: where to locate the restaurant? What kind of menu should be served at the restaurants that would be quite competitive to invite customers to the tables? How to go about hiring workers to help in the daily operations of the restaurant? What appropriate managerial policies to implement in order to successfully run the restaurant business? All these logistics requirements point to the fact that doing business in

Life of Pi Analysis Essay Example for Free

Life of Pi Analysis Essay Having just experienced the sinking of his family’s ship, and being put onto a life boat with only a hyena, Pi felt completely lost and alone. Then he met a familiar face from his family’s zoo, Pi saw the Bengal Tiger named Richard Parker onboard. His first reaction was to save the life of Richard Parker so that he may have a companion, and a protector aboard the lifeboat. Suddenly Pi realizes just what he is doing. He is saving the life of Richard Parker, by welcoming him, a 450 pound Bengal tiger, onto the small lifeboat. He experiences a change of heart when helping the tiger onto the boat. Pi realizes that he is now posing a threat on his own life. With Richard Parker on the boat, Pi is faced with not only the fight to survive stranded in the middle of the Pacific Ocean, but the fight to survive living with a meat eating tiger. The change of heart that Pi experiences might possibly mean that he is an impulsive thinker. It may mean that he often does something on impulse without thinking it through, and then later regrets his actions. Though he was having bad experiences onboard, Pi maintains his religious beliefs while on the life boat through his daily prayers. He takes time aside each day to say the prayers that he always would say. In one instance, he turns where he believes Mecca is located, and prays his traditional prayers towards Mecca. Pi also often states that he will include specific animals in his prayers, such as the zebra aboard his lifeboat, and the first fish that he ever killed. With Pi keeping his ritual prayers going, it helped him to survive. He made comparisons between that of Orange Juice, the orangutan, and the Virgin Mary, and would also often bring up Jesus on the cross. Truly, Pi’s religious faith remained strong throughout his journey on the Pacific Ocean.

Business Ethics And Cross Cultural Management Commerce Essay

Business Ethics And Cross Cultural Management Commerce Essay Ethics, or more accurately, the lack of ethics, on the part of business and government leaders has lately received significant attention in the mass media and has been a topic of discussion among the public, as well as the different organizations in the world (Peppas 2002). Business ethics has gained the attention of many scholars and executives in both private and public sectors because of many unethical practices that are being reported. This includes the cases of large multinational organizations including Enron (Rashid Ibrahim 2007). This is the reason why many studies about cultural differences and business ethics have been implemented in the past years. This literature review is conducted in order to analyze and evaluate the arguments regarding the different aspects that are related with business ethics and differences in cultures. Implications of Business Ethics The concept about ethics is considered as a complex matter because it is predicated on an interchange of views regarding the belief systems of individual among the citizens of any culture (Svensson Wood 2003). The study of business ethics and its connection for the stakeholders of organizations have experienced fast growth in the past few decades. The literature about business ethics is divided on its views regarding the motivation and the cause for organizations to have their ethical dimension. The study of Harrison (2001) showed that there are two main schools of thoughts first are those who suggest that firms are operating in order to generate profit, therefore, business ethics is considered as another means of attracting the customers, while the second are those who supported some corporate conscience as well as inherent motivation for the implementation of business ethics. On the other hand, Paul (2001) considered business ethics are very subjective in nature because it is a function of time and culture therefore, the business ethics have changed, together with the cultural values and norms motivated the business ethics in the national and regional boundaries. The study of Hofstede (1983) is considered as one of the most important studies which show the differences of countries or nations in terms of cultures and values. The study showed that there are 4 vital indicators which include individualism, power distance, uncertainty avoidance and masculinity which shows great differences in the culture among different nations. The study of Vinten (1991) divided the issues of business ethics in different degree, which include international business, domestic business and professional ethics. Each level constitute for different level of some unethical activities perform by organizations and businesses. Even though, there are many studies which show importance of ethics, and even though it is being followed by different organizations, there are still many reasons and disapproval of adoption of ethics in the world of business. This is connected to the study of Stemberg (1994) which focuses on the case of rejected relativism. It stated that ethics is seen as excessively theoretical, at the same time, contradict the primary reason of any business and it is also lacking of direction in terms of harmony across the different cultures. Business Ethics and Cultural Differences One of the challenges that business ethics is facing is the impact of globalization. As a result, it is common nowadays, for business managers and employees to work with and for people from different nations and races which represents many different cultural values. It is important to consider that culture affects virtually all of the aspects of the business relationship. This is because one aspect, that of business ethics, can cause huge conflict and gross misunderstanding between individuals who do not share the same backgrounds (Sims 2006). With this, the attitudes of individual towards the business ethics may be different greatly even within one culture that trying to come to an agreement across cultures can become nearly impossible. Thus, it may be that an agreement on ethics across cultures is not even recommended (Donaldson Dunfee 1999). Furthermore, in culturally homogenous groups, there is a greater possibility that values of individual members of the team will match. Howev er, because the because of the growing number of cross-cultural organizations, it is important to focus on this subject. Business ethics can be seen as a function of culture because the evolution of culture in the environment of the business will surely affect the acceptable and unacceptable actions inside and outside the organization. Different studies across cultures have been performed in the literature of ethics on the international level such as: Jakubowski et al., 2002; Fisher et al., 2001; Seitz, 2001; and Singhapakdi et al., 1999; and the national level which include Peppas Peppas, 2000; and Fernandez-Fernandez et al., 1999 (Svensson Wood 2003). The study of Singhapakdi Rawwas (1999) shows that the business ethics are considered as essential in the international business, because the culture of individual affect their ethical decisions. It presented that there are some vital differences between the countries, thus it is important to focus on those in order to ensure success of organizations. In connection, Vinten (1998) argues that business ethics has the possibility to become a vital factor of corporate strategy and culture, thus it must be considered in internal and external ethical audit. On the other hand, the study of Hood and Longsdon (2002) showed the similarities and differences of cultural values of managers in three countries. The result showed that one of the vital components of culture is ethics. Therefore, this factor of culture survives as a possible strong influence towards the success of the organizations in the entire marketplace. Bucar Glas (2002) expand a conceptual framework for the evaluation of the cross-cultural differences in the ethical attitudes of business people that are based on the theory of social contract. The result of the study showed that prognostic of the degree of the ethical attitudes among the societies and they focus on the more delicate influence of many social institutions on the ethical attitudes of different groups within the society. On the other hand, according to Sen (1997), in the concept of the role of cultures in the m otivating norms of the behavior of business, it is important to be familiar with the compound structures of principles of business, at the same time, focuses on the wide-ranging contact of sentiments. Business Ethics Theory and Practice Gap The article of Stark (1993) presented the gap between the theory and practice in business ethics. According to Stark (1993) academic researches about business ethics failed to present useful guidance towards the managerial decision-making regarding the different ethical issues. Particularly, this applies on the added urgency that some of the most complex ethical issues which happen in the international arena. This was supported by Dean (1998) who stated that the field of business ethics must also include a service aspect if it is to be considered as the profession of business ethics. This focuses on the qualification of business ethics as profession. Thus, the question lies on the capability of the business ethics as the leading or conducting behavior in the business world, thus, it pertains on whom the field of business ethics is focusing. In addition, the international business ethics can only be deemed a profession based on its tangible and existing advantage towards the actual or real-world risks and threats. Conclusion Business ethics is a vital aspect in any organization and business. This is because it enables them to focus on planning and implementing rules and regulations, standards and policies that will be beneficial to everyone by applying what is right and what is with accordance to the law. However, because of globalization, business ethics is facing different criticism and possible problem. This is because of the fact that culture affects how an individual or group perceives one thing or situation as good or bad. Therefore, it is important to focus on the different cultural factors in order to ensure future success of business world. Annotated Bibliography Morgan, E 1998, Navigating Cross-Cultural Ethics: What Global Managers Do Right To Keep From Going Wrong, Butterworth-Heinemann. This is a textbook which shows the personal stories of managers who are running global business. It focuses on analyzing the different dilemmas that global managers are facing in terms of ethical leadership in international business. Svensson, G Wood, G 2003, The Dynamics of Business Ethics: a Function of Time and Culture Case and Models, Management Decision, vol. 41, no. 4, pp. 350 361. This is a journal which examines the construct of ethics as a whole and of the business ethics in specific manner. Furthermore, it also offers a theoretical discussion of the dynamics of ethics in the society and in the marketplace. Gannon, M Newman, K 2002, The Blackwell Handbook of Cross-Cultural Management, Wiley-Blackwell. This textbook offer an overview of the different major research perspectives about cross-cultural management and ethics, it includes theories about ethics and cultures, together with how it can be applied in the real-world. Ferrell, O C, Fraedrich, J Ferrell, L 2006, Business Ethics: Ethical Decision Making and Cases, Cengage Learning. This textbook presents complex environment where in the managers face different ethical decision making. This include how cultural differences can affect how managers and members of the company perceive changes and decisions inside the company. Weiss, J 2008, Business Ethics: A Stakeholder and Issues Management Approach, Cengage Learning. This textbook presented the different issues that are related with the business ethics. This includes information about the impact of differences in cultures among different nations working in the business environment. Katsioloudes, M Hadjidakis, S 2007, International Business: A Global Perspective, Butterworth-Heinemann. This textbook enables to present the different theories and changes going on in the business environment in international arena. This will be helpful in order to locate information which pertains on the different challenges which global managers will be facing in terms of decision-making.

Reticence :: essays research papers

Reticence THIS MORNING there was a dead cat in the harbor, a black cat that was floating on the surface of the water. It was straight and stiff and was drifting slowly along the side of a boat. From its mouth dangled a decomposed fish head with a piece of broken line about two inches long coming out of it. At the time I had simply imagined that this fish head was what remained of the bait from a discarded fishing line. The cat must have leaned into the water to catch the fish and, as he grabbed it, the hook caught in his mouth and he lost his balance and fell. The water of the harbor was very dark where I was standing, but from time to time I suspected there was a procession of fish, mullets and wrasse, passing silently below my eyes, while on the bottom, among the pebbles and algae, thousands of wriggling fry worked over the gutted remains of a decomposing eel. Before setting off again, I lingered a moment longer on the pier staring at the dead cat still drifting in the harbor in a very slow back and forth movement, first to the left, then to the right, following the imperceptible ebb and flow of the current at the surface of the water. I had arrived in Sasuelo at the end of October. It was already autumn and the tourist season was coming to an end. A taxi dropped me off one morning, bag and baggage, on the village square. The driver helped me undo my son's stroller from the roof-rack of the car, an old 504 diesel that he left running and that continued to hum slowly in place. Then he pointed in the direction of the only hotel in the area, that I was familiar with since I had already stayed there. I left my bags near a bench and set off in the direction of the hotel with my son who was installed in his stroller in front of me and paying no attention to anything around him, absorbed as he was in the contemplation of a toy seal that he turned over and over in his hands examining all its seams and releasing for the occasion an imperturbable burp as naturally as a prince. At the entrance of the hotel flowers lined a little flight of steps at the top of which opened double French doors, and I took the stroller in my arms to climb the steps. Reticence :: essays research papers Reticence THIS MORNING there was a dead cat in the harbor, a black cat that was floating on the surface of the water. It was straight and stiff and was drifting slowly along the side of a boat. From its mouth dangled a decomposed fish head with a piece of broken line about two inches long coming out of it. At the time I had simply imagined that this fish head was what remained of the bait from a discarded fishing line. The cat must have leaned into the water to catch the fish and, as he grabbed it, the hook caught in his mouth and he lost his balance and fell. The water of the harbor was very dark where I was standing, but from time to time I suspected there was a procession of fish, mullets and wrasse, passing silently below my eyes, while on the bottom, among the pebbles and algae, thousands of wriggling fry worked over the gutted remains of a decomposing eel. Before setting off again, I lingered a moment longer on the pier staring at the dead cat still drifting in the harbor in a very slow back and forth movement, first to the left, then to the right, following the imperceptible ebb and flow of the current at the surface of the water. I had arrived in Sasuelo at the end of October. It was already autumn and the tourist season was coming to an end. A taxi dropped me off one morning, bag and baggage, on the village square. The driver helped me undo my son's stroller from the roof-rack of the car, an old 504 diesel that he left running and that continued to hum slowly in place. Then he pointed in the direction of the only hotel in the area, that I was familiar with since I had already stayed there. I left my bags near a bench and set off in the direction of the hotel with my son who was installed in his stroller in front of me and paying no attention to anything around him, absorbed as he was in the contemplation of a toy seal that he turned over and over in his hands examining all its seams and releasing for the occasion an imperturbable burp as naturally as a prince. At the entrance of the hotel flowers lined a little flight of steps at the top of which opened double French doors, and I took the stroller in my arms to climb the steps.

Homeless Cafes :: Essays Papers

Homeless Cafes There seems to be a welcome trend unfolding in social services for the homeless. In place of large, impersonal soup kitchens, small cafes are springing up with a philosophy that is all too rare-treat the homeless with respect and dignity, and maybe they'll respond to available services. It began with Cafe 458 in Atlanta in 1988, which since has served as a model for similar cafes across the country. Cafe 458 was founded to serve good meals to small groups of homeless individuals, offer a comprehensive array of services, and empower those who eat at the Cafe. Part of its strategy to empower homeless persons is to treat them with dignity-the staff refer to those who eat there as guests, not clients, food is served on real dishes, artwork adorns the walls, and fresh flowers brighten each table. The Cafe serves only 28 guests at a time, all of whom are referred by area social service agencies. To be referred to the Cafe, clients must not be actively using drugs, have no recent history of violence, and have a strong desire to improve their lives. On the average, guests regularly eat at the Cafe for six months before they are able to find employment and/or housing. Since 1988, Cafe 458 has served only 1,000 people, but the staff considers its small numbers its strength. As Nancy Palchanis, executive director, explains, "It is not a feeding program. It is about empowerment. Soup kitchens feed too many people. Our advantage is in the small size." The small size of Cafe 458, and others like it, is translating into success in the lives of their guests . For example, The Bread and Roses Cafe, run by a social service agency in Venice, California, enrolls some of its clients in a six-week intensive course in restaurant work and 20 hours of on-thejob training. Sixty-seven of the 112 people who have completed the program are now employed by restaurants in the Los Angeles area. Fortunately for some of Chicago's homeless, they too have a similar place to turn-Inspiration Cafe, located in the Uptown community. Since 1991, Inspiration Cafe has served good-tasting and healthy meals to homeless individuals in Uptown and has provided them with a variety of comprehensive services. The Cafe was founded by Lisa Nigro, a former homeless in her community. Nigro began feeding the homeless in Uptown by carting around a red wagon filled with bagels and juice that she would serve to people waiting in lines at local shelters and soup kitchens.

Chemical and Physical Properties Essay

Observe the solubility in hot water. c. Tear off approximately 1/2-cm pieces of both red and blue litmus paper. d. Allow the solution to cool, and using the glass stirring rod, transfer a drop of the solution onto a piece of litmus paper. Record the results. Clean the stirring rod. 5. Third test tube: a. Add a few pipet drops of HCl (hydrochloric acid) to the sample and stir. NOTE: Use the test tube holderclamp as some reactions are exothermic, i. e. the test tube may get very hot. Record any apparent reaction or solubility. Clean the stirring rod. 6. Fourth test tube: Add a few pipet drops of NaOH (sodium hydroxide) to the sample. Stir. Record any apparent reaction or solubility. Clean the stirring rod. 7. Thoroughly clean and dry all 4 test tubes. Repeat Steps 2 though 6 with the next substance. Experimental Results Substance: Zn Color: Grey Odor: None Effect of Heat: decreases content curled in with lighter color Solub. Or Reaction w/cold H20: No reaction observed / not soluble Solub. Or Reaction w/ hot H20: Prolonged boiling from retained heat/ non soluble Litmus test: None observed Dilute HCI: positive reaction resulting in the formation of gas, carbonation, and darkened Dilute NaOH: creates foggy substance and blackens solid Substance: Cu Color: Copper Odor: None noted Effect of Heat: Turned black Solub. Or Reaction w/cold H20: not soluble Solub. Or Reaction w/ hot H20: not soluble Litmus test: None observed Dilute HCI: tarnished not observed Dilute NaOH: none observed Substance: Mg Color: Silver Odor: None observed Effect of Heat: increases in dullness Solub. Or Reaction w/cold H20: no reaction observed/ non soluble Solub. Or Reaction w/ hot H20: increased heat / non soluble Litmus test: no change Dilute HCI: rapid boiling and heat Dilute NaOH: liquid is cloudy Substance: MgO Color: White Odor: None observed Effect of Heat: none observed Solub. Or Reaction w/cold H20: Soluble Solub. Or Reaction w/ hot H20: Soluble Litmus test: Red paper turned blue / blue no reaction Dilute HCI: Carbonated Soluble Dilute NaOH: Dense Soluble Substance: CuC03 Color: Green Odor: pungent Effect of Heat: turned black Solub. Or Reaction w/cold H20: slightly murky, but does not appear soluble Solub. Or Reaction w/ hot H20: slightly murky, but none soluble Litmus test: changes blue to red showing slight acidity Dilute HCI: carbonation and soluble Dilute NaOH: turns blue w/ slight soluble Substance: Cu(NO3)2 Color: Blue crystals Odor: slight odor Effect of Heat: melted and turned green Solub. Or Reaction w/cold H20: turned water blue and is soluble Solub. Or Reaction w/ hot H20: no change observed from the cool water and is soluble Litmus test: blue litmus turned to red for acid Dilute HCI: turned green Dilute NaOH: soluble formed Substance: NaCl Color: white crystals Odor: none observed Effect of Heat: none noted Solub. Or Reaction w/cold H20: becomes slightly soluble Solub. Or Reaction w/ hot H20: fully soluble Litmus test: red turns to blue indicating that it’s basic Dilute HCI: none observed Dilute NaOH: none observed QUESTIONS: A – Did you observe any chemical changes in this experiment? Chemical changes are observed during the experiment B – What evidence did you use to decide that something was a chemical change? Indications of chemical changes were preceded by the burning, rusting, ermenting, and decomposing of the substances. As a result, a new substance will have its own unique physical properties C – Give at least two examples of chemical changes you observed. Adding colorless sodium hydroxide to blue copper(II) nitrate produces a light blue precipitate Zinc diluted with Hydrogen Chloride is positive for a chemical reaction resulting in the formation of gas, carbonation, and darkened D à ¢â‚¬â€œ Classify the following properties of sodium metal as physical or chemical: Silver metallic color – physical Turns gray in air – chemical Melts at 98oC – physical Reacts explosively with chlorine – chemical E – Classify the following changes as physical or chemical: Water freezes at OoC – physcical change Baking soda when combined with vinegar produces bubbles:- chemical change Mothballs gradually disappear at room temperature – physical change Ice cubes in a freezer get smaller with time – physical change Baking soda loses mass as it is heated – physical chemical Tarnishing of silver – chemical change F – How would you show that dissolving table salt is a physical change? A physical change will not change the composition of a substance. When adding water to a salt, the ions of table salt are hydrated with water molecules which reduced the strong electrostatic forces of the ions. To return salt to the original state, simply remove the hydrated water molecules by boiling the table salt. Conclusions: In a chemical change one or more new substances are formed. The new substance has its own unique identity which is different from the original. It has properties that are different than those of the starting material; in addition, the original materials is not able to be reproduced by physical means.